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joung lab real assembly talen kit  (Addgene inc)


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    Structured Review

    Addgene inc joung lab real assembly talen kit
    Joung Lab Real Assembly Talen Kit, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/joung+lab+real+assembly+talen+kit/REAL+Assembly+TALEN+Kit+(Kit+%231000000017)/pm37923055-82-9-15
    Average 93 stars, based on 33 article reviews
    joung lab real assembly talen kit - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: XRCC4 and MRE11 Roles and Transcriptional Response to Repair of TALEN-Induced Double-Strand DNA Breaks
    Article Snippet: After 6 h of incubation, media was replaced, and cells were cultured in complete media for 48 h. Cell supernatant were collected, filtered through a 0.45 μm syringe filter (MilliporeSigma, Burlington, MA, USA), and used for transduction into HeLa cells. .. TALEN expression constructs (TAL 256 and TAL 278) were previously constructed with the Joung Lab REAL Assembly TALEN kit (Addgene, Watertown, MA, USA) [ , ]. .. To assess DNA repair, HeLa cells (0.6 million) were seeded a day before transient transfection, co-transfected with a mCherry/GFP reporter plasmid and pairs of TALEN-expressing constructs [TAL 256 (200 ng) and TAL 278 (200 ng)] at a ratio of 1:3 [DNA (μg): Transfection reagent (μL)] with Viafect transfection reagent (Promega, Madison, WI, USA).

    Construct:

    Article Title: XRCC4 and MRE11 Roles and Transcriptional Response to Repair of TALEN-Induced Double-Strand DNA Breaks
    Article Snippet: After 6 h of incubation, media was replaced, and cells were cultured in complete media for 48 h. Cell supernatant were collected, filtered through a 0.45 μm syringe filter (MilliporeSigma, Burlington, MA, USA), and used for transduction into HeLa cells. .. TALEN expression constructs (TAL 256 and TAL 278) were previously constructed with the Joung Lab REAL Assembly TALEN kit (Addgene, Watertown, MA, USA) [ , ]. .. To assess DNA repair, HeLa cells (0.6 million) were seeded a day before transient transfection, co-transfected with a mCherry/GFP reporter plasmid and pairs of TALEN-expressing constructs [TAL 256 (200 ng) and TAL 278 (200 ng)] at a ratio of 1:3 [DNA (μg): Transfection reagent (μL)] with Viafect transfection reagent (Promega, Madison, WI, USA).

    Article Title: Compositions comprising TALENs and methods of treating HIV
    Article Snippet: .. Plasmid DNA constructs for the HT-TALENs were built using the Joung Lab REAL Assembly TALEN kit (AddGene), following the REAL Assembly method as described (Miller et al. (2011) Nat Biotechnol 29: 143-148). .. Identity of correct HT-TALEN DNA clones was confirmed by sequence analysis (Beckman Coulter Genomics).

    Article Title: Zebrafish model of human Zellweger syndrome reveals organ-specific accumulation of distinct fatty acid species and widespread gene expression changes.
    Article Snippet: .. TAL effector nucleases (TALENs) were designed using ZiFiT (http:// zifit.partners.org/ZiFiT/) and constructed using the Joung Lab REAL Assembly TALEN kit (a gift from Keith Joung; Addgene, Cambridge, MA, #1000000017), according to themanufacturer's protocol [65]. ..

    Article Title: Zebrafish model of human Zellweger syndrome reveals organ specific accumulation of distinct fatty acid species and widespread gene expression changes
    Article Snippet: To increase viability, zebrafish larvae were fed Paramecium tetraurelia and Paramecium sp . (obtained from the National BioResource Project, Paramecium; Yamaguchi University, Yamaguchi, Japan) raised in barley grass extract (made from young barley grass powder; Yamamoto Kanpoh Pharmaceutical, Aichi, Japan), supplemented with Klebsiella pneumoniae (a gift from Dr. Hideo Dohra, Shizuoka University). .. TAL effector nucleases (TALENs) were designed on ZiFiT ( http://zifit.partners.org/ZiFiT/ ) and constructed using the Joung Lab REAL Assembly TALEN kit (a gift from Keith Joung; Addgene, Cambridge, MA, #1000000017), according to the provided protocol ( ). ..

    Plasmid Preparation:

    Article Title: Compositions comprising TALENs and methods of treating HIV
    Article Snippet: .. Plasmid DNA constructs for the HT-TALENs were built using the Joung Lab REAL Assembly TALEN kit (AddGene), following the REAL Assembly method as described (Miller et al. (2011) Nat Biotechnol 29: 143-148). .. Identity of correct HT-TALEN DNA clones was confirmed by sequence analysis (Beckman Coulter Genomics).

    TALENs:

    Article Title: Zebrafish model of human Zellweger syndrome reveals organ-specific accumulation of distinct fatty acid species and widespread gene expression changes.
    Article Snippet: .. TAL effector nucleases (TALENs) were designed using ZiFiT (http:// zifit.partners.org/ZiFiT/) and constructed using the Joung Lab REAL Assembly TALEN kit (a gift from Keith Joung; Addgene, Cambridge, MA, #1000000017), according to themanufacturer's protocol [65]. ..

    Article Title: Reducing CXCR4 expression and/or function to enhance engraftment of hematopoietic stem cells
    Article Snippet: .. In addition, commercially available kits can be used to design and develop vectors that include TALENs specific for a target (e.g., Golden Gate TALEN and TAL Effector Kit from Addgene (Cambridge, Mass.), catalog #1000000024 and Joung Lab REAL Assembly TALEN kit, from Addgene, catalog #1000000017). ..

    Article Title: Zebrafish model of human Zellweger syndrome reveals organ specific accumulation of distinct fatty acid species and widespread gene expression changes
    Article Snippet: To increase viability, zebrafish larvae were fed Paramecium tetraurelia and Paramecium sp . (obtained from the National BioResource Project, Paramecium; Yamaguchi University, Yamaguchi, Japan) raised in barley grass extract (made from young barley grass powder; Yamamoto Kanpoh Pharmaceutical, Aichi, Japan), supplemented with Klebsiella pneumoniae (a gift from Dr. Hideo Dohra, Shizuoka University). .. TAL effector nucleases (TALENs) were designed on ZiFiT ( http://zifit.partners.org/ZiFiT/ ) and constructed using the Joung Lab REAL Assembly TALEN kit (a gift from Keith Joung; Addgene, Cambridge, MA, #1000000017), according to the provided protocol ( ). ..

    Article Title: A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing
    Article Snippet: PCR products were run on a 1% agarose, gel, gel purified and digested with SbfI and NheI (upstream homologous arm), and AscI and NotI (downstream homologous arm), respectively, and ligated into the OCT4-2A-eGFP-PGK-Puro vector (Addgene; plasmid 31938) replacing the OCT4 homologous arms. .. TALENs targeting 5’-TGGCTAATGC CCTGGCC-3’ upstream of the HBB stop codon, and 5’-TGGACAGCAA GAAAGCGAGC-3’ downstream of the HBB stop codon, respectively, were assembled using the Joung Lab REAL Assembly TALEN kit (Addgene; Kit # 1000000017) according to protocol by . ..



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    93
    Addgene inc joung lab real assembly talen kit
    Joung Lab Real Assembly Talen Kit, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/joung+lab+real+assembly+talen+kit/REAL+Assembly+TALEN+Kit+(Kit+%231000000017)/pm37923055-82-9-15
    Average 93 stars, based on 1 article reviews
    joung lab real assembly talen kit - by Bioz Stars, 2026-10
    93/100 stars
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